Second life of water and wastewater in the context of circular economy - Do the membrane bioreactor technology and storage reservoirs make the recycled water safe for further use?

In recent years water demand drastically increased which is particularly evident in tourism-burdened mountain regions. In these areas, climate neutral circular economy strategies to minimize human impact on the environment can be successfully applied. Among these strategies, treated wastewater reuse and retaining water in storage reservoirs deserve particular attention. This study aimed to determine if recycled water produced with two circular economy systems, namely membrane bioreactor treatment plant (MBR) with UV-light effluent disinfection and a storage reservoir, is safe enough for further use in green areas irrigation in summer and artificial snow production in winter. The assessment was based on the presence and concentration of antimicrobial agents, antibiotic resistant bacteria, antibiotic resistance genes, bacterial community composition and diversity. The treated water and wastewater was compared with natural water in their vicinity. Both systems fulfill the criteria set by the European Union in terms of reclaimed water suitable for reuse. Although the MBR/UV light wastewater treatment substantially reduced the numbers of E. coli and E. faecalis (from e.g. 32,000 CFU/100 ml to 20 CFU/100 ml and 15,000 CFU/100 ml to nearly 0 CFU/ml), bacteria resistant to ampicillin, aztreonam, cefepime, ceftazidime, ertapenem and tigecycline, as well as ESBL-positive and multidrug resistant E. coli were highly prevalent in MBR-treated wastewater (88.9 %, 55.6 %, 33.3 %, 22.2 % and 11.1 % and 44.4 and 55.6 %, respectively). Applying additional tertiary treatment technology is recommended. Retaining water in storage reservoirs nearly eliminated bacterial contaminants (e.g. E. coli dropped from 350 CFU/100 ml to 10 CFU/100 ml), antibiotic resistant bacteria, resistance genes (none detected in the storage reservoir) and antibiotics (only enrofloxacin detected once in the concentration of 3.20 ng/l). Findings of this study point to the limitations of solely culture-based assessment of reclaimed water and wastewater while they may prove useful in risk management and prevention in wastewater reuse.

EUSEDcollab: a network of data from European catchments to monitor net soil erosion by water.

As a network of researchers we release an open-access database (EUSEDcollab) of water discharge and suspended sediment yield time series records collected in small to medium sized catchments in Europe. EUSEDcollab is compiled to overcome the scarcity of open-access data at relevant spatial scales for studies on runoff, soil loss by water erosion and sediment delivery. Multi-source measurement data from numerous researchers and institutions were harmonised into a common time series and metadata structure. Data reuse is facilitated through accompanying metadata descriptors providing background technical information for each monitoring station setup. Across ten European countries, EUSEDcollab covers over 1600 catchment years of data from 245 catchments at event (11 catchments), daily (22 catchments) and monthly (212 catchments) temporal resolution, and is unique in its focus on small to medium catchment drainage areas (median = 43 km2, min = 0.04 km2, max = 817 km2) with applicability for soil erosion research. We release this database with the aim of uniting people, knowledge and data through the European Union Soil Observatory (EUSO).

Anthropogenic pollution gradient along a mountain river affects bacterial community composition and genera with potential pathogenic species.

Mountain regions in Poland are among the most frequently visited tourist destinations, causing a significant anthropogenic pressure put on the local rivers. In this study, based on numbers of 9 microorganisms, content of 17 antibiotics and 17 physicochemical parameters, we determined a pollution gradient in six sites along Bialka, a typical mountain river in southern Poland. The E.coli/Staphylococcus ratio varied evidently between polluted and non-polluted sites, indicating that the possible utility of this parameter in assessing the anthropogenic impact on river ecosystems is worth further investigation. Then, using next generation sequencing, we assessed the changes in bacterial community structure and diversity as a response to the pollution gradient. Proteobacteria and Bacteroidetes were the most abundant phyla in the majority of samples. Actinobacteria were the most abundant in the most pristine (groundwater) sample, while Firmicutes and Verrucomicrobia were more prevalent in polluted sites. Bacterial diversity at various levels increased with water pollution. Eleven bacterial genera potentially containing pathogenic species were detected in the examined samples, among which Acinetobacter, Rhodococcus, and Mycobacterium were the most frequent. At the species level, Acinetobacter johnsonii was most prevalent potential pathogen, detected in all surface water samples, including the pristine ones. Two bacterial taxa-genus Flectobacillus and order Clostridiales showed very distinct variation in the relative abundance between the polluted and non-polluted sites, indicating their possible potential as biomarkers of anthropogenic impact on mountain river waters.

COVID-19 lockdown shows how much natural mountain regions are affected by heavy tourism.

Mountain areas in Poland are among the most frequented tourist destinations and such intensive tourism negatively affects the natural environment. The COVID-19 pandemic and the resulting lockdown restricted travel for a few months in 2020, providing a unique opportunity to observe the studied mountain environment without the impact of typical tourist traffic. This study is based on the determination of antibiotic content, hydrochemical parameters, enumeration of culturable bacterial water quality indicators, antimicrobial susceptibility tests together with extended spectrum beta-lactamase (ESBL) gene detection in waterborne E. coli and NGS-based bacterial community composition at six sites along the Bialka river valley (one of the most popular touristic regions in Poland) in three periods: in summer and winter tourist seasons and during the COVID-19 lockdown. The results of individual measurements showed decreased numbers of bacterial indicators of water contamination (e.g. numbers of E. coli dropped from 99 × 104 CFU/100 ml to 12 CFU/100 ml at the most contaminated site) and the share of antimicrobial resistant E. coli (total resistance dropped from 21% in summer to 9% during lockdown, share of multidrug resistant strains from 100 to 44%, and ESBL from 20% in summer to none during lockdown). Antibiotic concentrations were the highest during lockdown. The use of multivariate analysis (principal component analysis - PCA and heatmaps) revealed a clear pattern of tourism-related anthropogenic pressure on the water environment and positive impact of COVID-19 lockdown on water quality. PCA distinguished three major factors determining water quality: F1 shows strong effect of anthropogenic pressure; F2 describes the lockdown-related quality restoration processes; F3 is semi-natural and describes the differences between the most pristine and most anthropogenically-impacted waters.

Impact of ski run construction on atypical channel head development.

The development of ski infrastructure in mountainous areas has a significant impact on the development of erosion landforms and modification of existing landforms on slopes. The main purpose of our study was to determine the effects of ski run construction and artificial snowmaking on the development of channel heads and drainage lines as well as pattern of processes occurring along longitudinal profile of stream channel. The research was performed in the Remiaszów catchment on two ski runs in the Bialczanska Kotelnica ski area (the Polish Inner Carpathians). Analysis of the location of the channel head of the Remiaszów Stream catchment over time was performed on the basis of multi-temporal DEMs generated using high resolution LiDAR data. The data were obtained via two ALS scans (Airborne Laser Scanning): before the 2013 and after 2016 ski run construction, as well as TLS (Terrestrial Laser Scanning) conducted in 2017 and in the course of fieldwork in 2018 using GPS RTK. The construction of ski runs leads to the formation of new channel heads, all of which develop in drainage ditches. Three distinct zones emerge in the longitudinal profile: 1) artificially concentrated flow zone, 2) forced, dispersed flow zone, 3) rejuvenated, concentrated flow zone. This means that two channel head formation zones become available in the longitudinal profile. Large inflows of water (200% or more) due to artificial snowmaking lead to bidirectional development of the valley network. In general, identification of the channel head pattern in area of ski run construction play important role in understanding of the sustainable development of mountain ecosystem.

Intra-annual groundwater levels and water temperature patterns in raised bogs affected by human impact in mountain areas in Poland.

Over the last century, the vast majority of peatlands in Europe have experienced substantial transformation as a result of drainage works that led to an imbalance in the natural hydrologic regime as well as changes in vegetation composition. The ongoing study aims to reconstruct the natural hydrologic regime of peatlands and restore their typical vegetation communities. In this study, we examine the variability of groundwater levels and groundwater temperature in raised bogs located in the Bieszczady Mts. in southern Poland. Both groundwater table levels and groundwater temperature serve to characterise the hydrology of peatlands, which in turn is critical for plant growth and rates of relevant biochemical processes. Our objective is to determine the predominant scale of intra-annual variability in time series and identify their potential sources by assessing the adaptive response of peat bogs to key changes in weather conditions. For this purpose, data obtained from 9 monitoring wells located in peat bogs, with a varying degree of degradation, were used. Fluctuations in time series and potential linkages between selected variables were analysed in the frequency domain using the continuous wavelet transform. The results show that peat bogs exhibit a relatively high stability of groundwater table levels and groundwater temperature despite meaningful changes in weather conditions. The most visible response of peat bogs to weather conditions was observed in summer and autumn. Our study demonstrates that degraded peat bogs experience the largest decrease in groundwater table levels and more frequent fluctuations. In contrast, groundwater temperature remained stable throughout the year at all the studied bog sites.

Effect of the construction of ski runs on changes in relief in a mountain catchment (Inner Carpathians, Southern Poland).

In the last decade increasing popularity of winter tourism in mountain areas in Poland influenced development of ski infrastructure. This type of human activity may induce changes in mountain relief. The purpose of the study was to quantify ongoing change patterns via: (i) a determination of spatial and quantitative changes in catchment covered by new ski runs, (ii) a determination of the effect of new ski runs on the rejuvenation of relief in valleys adjacent to ski runs, (iii) an identification of changes in the surface runoff pattern before and after the construction of ski runs. The research was carried out in the Remiaszów catchment on two ski runs (southern Poland). Airborne Laser Scanning (ALS) data from 2013 and 2016 were also used in the study along with Terrestrial Laser Scanning (TLS) data from 2015. LiDAR (Light Detection and Ranging) point clouds were interpolated to create multi-temporal DEMs and then these DEMs were used to derive DoDs. These were used to identify erosion and accumulation zones. The Convergence Index (CI) was used to determine the direction of surface runoff. The largest changes in relief were observed in areas with ski runs, with ski run E lowering an average of 0.07m (±0.03m), and ski run N an average of 0.12m (±0.03m). The entire area lowered about 0.02m. The construction of new ski runs resulted in a rejuvenation of denudation valleys located in the vicinity of existing ski runs. Valley incisions reaching 1.5m (±0.15m) were observed. Both the convergence and divergence zones for surface runoff were identified, which made it possible to show changes in the geometry of flow direction. The identification of these sites may help forecast erosion and deposition zones. In general, this may make it easier to identify areas substantially susceptible to relief change.

Effect of Land Use, Seasonality, and Hydrometeorological Conditions on the K+ Concentration-Discharge Relationship During Different Types of Floods in Carpathian Foothills Catchments (Poland).

The purpose of the study was to determine the role of land use, seasonality, and hydrometeorological conditions on the relationship between stream water potassium (K+) concentration and discharge during different types of floods-short- and long-duration rainfall floods as well as snowmelt floods on frozen and thawed soils. The research was conducted in small catchments (agricultural, woodland, mixed-use) in the Carpathian Foothills (Poland). In the woodland catchment, lower K+ concentrations were noted for each given specific runoff value for summer rainfall floods versus snowmelt floods (seasonal effect). In the agricultural and mixed-use catchments, the opposite was true due to their greater ability to flush K+ out of the soil in the summer. In the stream draining woodland catchment, higher K+ concentrations occurred during the rising limb than during the falling limb of the hydrograph (clockwise hysteresis) for all flood types, except for snowmelt floods with the ground not frozen. In the agricultural catchment, clockwise hystereses were produced for short- and long-duration rainfall floods caused by high-intensity, high-volume rainfall, while anticlockwise hystereses were produced for short- and long-duration rainfall floods caused by low-intensity, low-volume rainfall as well as during snowmelt floods with the soil frozen and not frozen. In the mixed-use catchment, the hysteresis direction was also affected by different lag times for water reaching stream channels from areas with different land use. K+ hystereses for the woodland catchment were more narrow than those for the agricultural and mixed-use catchments due to a smaller pool of K+ in the woodland catchment. In all streams, the widest hystereses were produced for rainfall floods preceded by a long period without rainfall.

Effect of water stage and tree stand composition on spatiotemporal differentiation of spring water chemistry draining Carpathian flysch slopes (Gorce Mts).

The purpose of this study was to identify the factors affecting spring water chemistry in different tree stands and to measure the influence of water stage on the physicochemical parameters of spring waters in a small Carpathian catchment. Water samples were collected three times per year at various stages of the water: after the spring thaw, after a period of heavy rain and after a dry period in 2011 and 2012. Water samples were left in the laboratory to reach room temperature (19-20°C) and analyzed for EC (reference T=25°C) and pH. After filtration through 0.45µm PTFE syringe filters, the water samples were analyzed by means of ion chromatography using a DIONEX ICS 5000 unit. The following ions were analyzed: Ca2+, Mg2+, Na+, K+, HCO3-, SO42-, Cl-, and NO3-. Multivariate analysis (PCA) allowed the identification of two factors of spring water chemistry: factor 1, water stage and factor 2 tree stand composition. Seasonal variation of spring water chemistry showed that, higher pH values and mineralization as well as higher concentrations of Ca2+ and Mg2+ were measured during low water stage periods while lower EC and pH values were noted after spring snowmelt and rainfall, when higher concentrations of NO3- and SO42- were also found. Higher concentrations of Ca2+ and Mg2+ and higher pH of spring waters located in beech-fir stands and in those mixed with a large proportion of beech as well as a lower concentration of Ca2+, Mg2+ and HCO3-, pH, conductivity and mineralization of these spring waters, in which the alimentation areas were covered by upper subalpine spruce stands were noted.

The effect of anthropogenic pressure shown by microbiological and chemical water quality indicators on the main rivers of Podhale, southern Poland.

This study was aimed to determine the spatial variation in anthropogenic pressure exerted on surface water in the Podhale region (southern Poland), which is one of the most popular tourist destinations in Poland. The assessment was based on the dynamics and relationships between microbiological and chemical indicators of water quality throughout the major rivers of this region-Dunajec, Czarny Dunajec, Bialy Dunajec, and Bialka. Another aim was to assess the effect of land use on the quality of water in the studied rivers. The study was conducted over 1 year at 21 sampling sites distributed from the uppermost sections of rivers in the Tatra National Park through main tourist resorts until mouths of the considered rivers to the Czorsztynskie Lake. Microbiological analysis comprised the prevalence of total and fecal types of coliforms and Escherichia coli, mesophilic, and psychrophilic bacteria. Chemical assays determined the concentrations of Na+, K+, NH4+, Cl-, NO3-, and PO43-. Temperature, electrical conductivity, and pH were measured onsite. It was demonstrated that there is a significant relationship between the predominant types of land use within individual catchments, which results in evident differences in the pollution of waters between the catchments. The results showed that increased share of built-up areas and arable land results in significant deterioration of water quality. Thus, waters of Czarny Dunajec were the cleanest, while Bialy Dunajec was the most heavily contaminated. Also, spatial diversity in water quality was shown-the cleanest waters were sampled in the Tatra National Park and the pollution increased with the course of rivers. Point sources of pollution such as effluents from treatment plants or discharge of untreated sewage from households proved to be more important than non-point sources, such as surface runoff. Moreover, the important role of the Czorsztynskie Lake in the purification of water was demonstrated.

Factors and Mechanisms Affecting Seasonal Changes in the Prevalence of Microbiological Indicators of Water Quality and Nutrient Concentrations in Waters of the Bialka River Catchment, Southern Poland.

This 3-year study was aimed to understand the factors and mechanisms that cause the temporal changes in the concentration of microbiological indicators of water quality and nutrient concentration in selected sites of the Bialka river catchment (southern Poland) situated in direct vicinity of the largest ski station in the region. The analysis comprised 35 sampling campaigns conducted in five sites. Water temperature, pH, and electrical conductivity were measured during sampling, laboratory analyses included determination of the selected nutrients content (NH4, NO3, NO2, PO4); and the number of mesophilic and psychrophilic bacteria, coliforms, fecal coliforms, and Escherichia coli. Based on the cluster analysis, the collected samples were grouped into three to four groups, depending on the most characteristic features. Seasonal variation was evident, showing the predominance of either anthropogenic or natural-environment factors, depending on the considered season. On the other hand, principal component analysis revealed clear effect of various forms of land use in different sites.

Effect of deforestation on stream water chemistry in the Skrzyczne massif (the Beskid Slaski Mountains in southern Poland).

The purpose of the study was to identify the factors affecting stream water chemistry in the small mountain catchments deforested to varying degrees, from 98.7 to 14.1%, due to long-term acid deposition. Water samples were collected monthly in 2013 and 2014 from 17 streams flowing across three distinct elevation zones in the Skrzyczne massif (Poland): Upper, Middle and Lower Forest Zone. Chemical and physical analyses, including the pH, electrical conductivity (EC), total mineral content (Mt), water temperature, and the concentrations of Ca(2+), Mg(2+), Na(+), K(+), HCO3(-), SO4(2-), Cl(-), and NO3(-), were conducted. Based on Principal Component Analysis (PCA), the most important factor affecting water chemistry was human impact associated with changes in pH, SO4(2-) concentration, and the concentration of most of the main ions. The substantial acidity of the studied environment contributed to the exclusion of natural factors, associated with changes in discharge, from the list of major factors revealed by PCA. All of the streams were characterized by very low EC, Mt, and low concentrations of the main ions such as Ca(2+) and HCO3(-). This is the effect of continuous leaching of solutes from the soils by acidic precipitation. The lowest parameter values were measured for the streams situated in the Upper Forest Zone, which is associated with greater acid deposition at the higher elevations. In the streams located in the Upper Forest Zone, a higher percentage of SO4(2-) occurred than in the streams situated in the Middle and Lower Forest Zones. However, the largest share of SO4(2-) was measured in the most deforested catchment. The saturation of the studied deforested catchment with sulfur compounds is reflected by a positive correlation between SO4(2-) and discharge. Hence, a forest acts as a natural buffer that limits the level of acidity in the natural environment caused by acidic atmospheric deposition.

Spatiotemporal dynamics of spring and stream water chemistry in a high-mountain area.

The present study deals with the application of the self-organizing map (SOM) technique in the exploration of spatiotemporal dynamics of spring and stream water samples collected in the Chocholowski Stream Basin located in the Tatra Mountains (Poland). The SOM-based classification helped to uncover relationships between physical and chemical parameters of water samples and factors determining the quality of water in the studied high-mountain area. In the upper part of the Chocholowski Stream Basin, located on the top of the crystalline core of the Tatras, concentrations of the majority of ionic substances were the lowest due to limited leaching. Significantly higher concentration of ionic substances was detected in spring and stream samples draining sedimentary rocks. The influence of karst-type springs on the quality of stream water was also demonstrated.